Compatibility of the entomopathogenic fungus Beauveria bassiana with the insecticides fipronil, pyriproxyfen and hexaflumuron

Authors

  • A. Sheikhi Garjan
  • B. Naseri
  • H. Barari
  • M. Ghazavi
  • M. Rashid
Abstract:

سازگاری سه حشره‌کش فیپرونیل، پایری‌پروکسی‌فن و هگزافلومورون با جدایه‌ی DEBI 002 (آتشگاه) قارچ Beauveria bassiana مورد بررسی قرار گرفت. برای تعیین اثر این حشره‌کشها روی جوانه‌زنی اسپور قارچ، غلظت‌های مختلف آن‌ها به محیط کشت مالت آگار اضافه شد. به‌منظور محاسبه‌ی میزان رشد میسیلیوم در تیمارهای مختلف، قطر کلنی اندازه‌گیری شد و ارزیابی میزان اسپورزایی با شمارش اسپورها در واحد سطح صورت گرفت. نتایج نشان داد که پایری‌پروکسی‌فن در غلظت 1500 پی‌پی‌ام و هگزافلومورون در غلظت 800 پی‌پی‌ام به‌طور کامل مانع رشد میسیلیوم شدند، درحالی‌که میزان بازدارندگی فیپرونیل در غلظت 1600 پی‌پی‌ام، 6/76% بود. تأثیر بازدارندگی هر سه حشره‌کش در غلظت‌های پایین‌تر، 20-10% بود. میزان مهار اسپورزایی هر سه حشره‌کش در بالاترین غلظت تفاوت معنی‌داری با یکدیگر نداشت و 100-80% بود. پایری‌پروکسی‌فن در غلظت 400 پی‌پی‌ام و هگزافلومورون در تمام غلظت‌ها مانع جوانه‌زنی اسپور شدند و درصد جوانه‌زنی این تیمارها با سایر تیمارها تفاوت معنی‌داری داشت. براساس نتایج، هگزافلومورون بیشترین اثر بازدارندگی را روی جوانه‌زنی اسپورها داشت، و بنابراین، کاربرد هم‌زمان آن با قارچ بیمارگر B. bassiana در کنترل آفات توصیه نمی‌شود.

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Journal title

volume 31  issue 2

pages  29- 37

publication date 2012-02-20

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